期刊
JOURNAL OF VIROLOGICAL METHODS
卷 153, 期 2, 页码 232-237出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2008.06.027
关键词
CBPV; RNA virus; ISH; Honey bee brain; Genomic and antigenomic RNA detection
资金
- French Ministere de l'agriculture et de l'alimentation
Chronic bee paralysis virus (CBPV) is a common single-stranded RNA virus which may cause significant losses in honey bee colonies. As this virus seems to exhibit neurotropism, an in situ hybridization based method was developed to localize the genomic and antigenomic CBPV RNAs in infected honey bee brains. Double-stranded cDNA probes as well as genomic and antigenomic-specific single-stranded cDNA probes were prepared, using the polymerase chain reaction in presence of labelled d-UTP with non-radioactive digoxigenin. Both genomic and antigenomic RNAs were detected the brain of honey bee infected naturally or artificially. Hybridization signals were obtained in some somata and neuropile regions of the brain. In particular, high signals were observed at the level of the mushroom bodies and central complex, regions that are known to be engaged in higher neuronal functions and in the optic and antennal lobes that are sensorial neuropiles. Thus, the presence of virus at these levels may explain the nervous symptoms observed in infected bees. The in situ hybridization procedure proved to be a useful tool to localize specifically CBPV and may be helpful for understanding the observed symptoms. (C) 2008 Elsevier B.V. All rights reserved.
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