期刊
JOURNAL OF VIRAL HEPATITIS
卷 22, 期 3, 页码 281-288出版社
WILEY-BLACKWELL
DOI: 10.1111/jvh.12291
关键词
HCV; HIV; intrahepatic immunity; T cell
资金
- National Institute of Allergy and Infectious Diseases
Accelerated intrahepatic hepatitis C virus (HCV) pathogenesis is likely the result of dysregulation within both the innate and adaptive immune compartments, but the exact contribution of peripheral blood and liver lymphocyte subsets remains unclear. Prolonged activation and expansion of immunoregulatory cells have been thought to play a role. We determined immune cell subset frequency in contemporaneous liver and peripheral blood samples from chronic HCV-infected and HIV/HCV-coinfected individuals. Peripheral blood mononuclear cells (PBMC) and biopsy-derived liver-infiltrating lymphocytes from 26 HIV/HCV-coinfected, 10 chronic HCV-infected and 10 HIV-infected individuals were assessed for various subsets of T and B lymphocytes, dendritic cell, natural killer (NK) cell and NK T-cell frequency by flow cytometry. CD8(+) T cells expressing the exhaustion marker PD-1 were increased in HCV-infected individuals compared with uninfected individuals (P=0.02), and HIV coinfection enhanced this effect (P=0.005). In the liver, regulatory CD4(+)CD25(+)Foxp3(+) T cells, as well as CD4(+)CD25(+)PD1(+) T cells, were more frequent in HIV/HCV-coinfected than in HCV-monoinfected samples (P<0.001). HCV was associated with increased regulatory T cells, PD-1(+) T cells and decreased memory B cells, regardless of HIV infection (P0.005 for all). Low CD8(+) expression was observed only in PD-1(+)CD8(+) T cells from HCV-infected individuals and healthy controls (P=0.002) and was associated with enhanced expansion of exhausted CD8(+) T cells when exposed in vitro to PHA or CMV peptides. In conclusion, in HIV/HCV coinfection, ongoing HCV replication is associated with increased regulatory and exhausted T cells in the periphery and liver that may impact control of HCV. Simultaneous characterization of liver and peripheral blood highlights the disproportionate intrahepatic compartmentalization of immunoregulatory T cells, which may contribute to establishment of chronicity and hepatic fibrogenesis in HIV coinfection.
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