期刊
JOURNAL OF VETERINARY SCIENCE
卷 12, 期 1, 页码 41-48出版社
KOREAN SOC VETERINARY SCIENCE
DOI: 10.4142/jvs.2011.12.1.41
关键词
Aeromonas salmonicida; minimal inhibitory concentration; pulsed.field gel electrophoresis; quinolone-resistance determining region; tetracycline-resistance
资金
- Korean Research Foundation [KRF-2008-331-E00385]
- Ministry of Education, Science, and Technology [2009-0074437]
- National Research Foundation of Korea [2008-331-E00385, 2009-0074437] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The antibiotic resistance of 16 Aeromonas (A.) salmonicida strains isolated from diseased fish and environmental samples in Korea from 2006 to 2009 were investigated in this study. Tetracycline or quinolone resistance was observed in eight and 16 of the isolates, respectively, based on the measured minimal inhibitory concentrations. Among the tetracycline-resistant strains, seven of the isolates harbored tetA gene and one isolate harbored tetE gene. Additionally, quinolone-resistance determining regions (QRDRs) consisting of the gyrA and parC genes were amplified and sequenced. Among the quinolone-resistant A. salmonicida strains, 15 harbored point mutations in the gyrA codon 83 which were responsible for the corresponding amino acid substitutions of Ser(83)-> Arg(83) or Ser(83)-> Asn(83). We detected no point mutations in other QRDRs, such as gyrA codons 87 and 92, and parC codons 80 and 84. Genetic similarity was assessed via pulsed-field gel electrophoresis, and the results indicated high clonality among;he Korean antibiotic-resistant strains of A. salmonicida.
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