期刊
JOURNAL OF VETERINARY SCIENCE
卷 11, 期 2, 页码 151-154出版社
KOREAN SOC VETERINARY SCIENCE
DOI: 10.4142/jvs.2010.11.2.151
关键词
acridine orange; chromatin integrity; field fertility; porcine semen; sperm motility
An investigation involving seven boars, active in artificial insemination, and 1,350 multiparous sows was conducted at a private farm and aimed at examining the relationship between sperm quality traits and boar fertility in terms of farrowing rate and litter size. This experiment was done for 6 months. The semen samples were evaluated for subjective sperm motility and concentration. Ejaculates with at least 1 x 10(8) sperm/mL and 70% sperm progressive motility were extended with a commercial medium to 30 x 10(6) sperm/mL and used for artificial insemination (AI). AI dose was 100 mL semen containing 3 x 10(9) spermatozoa. Aliquots of diluted semen were assessed for live morphologically normal spermatozoa (LMNS, eosin-nigrosin stain exclusion assay) and sperm chromatin instability (SCI acridine orange assay). Farrowing rates according to different boar sperm varied (p < 0.001) from 59.3 to 88.92%. The mean values of LMNS (47.2 similar to 76.5%) and SCI (0.16 similar to 4.67%) differed significantly among boars. LMNS (r = 0.79, p < 0.05) and SCI (r = -0.90, p < 0.02) accounted for 62.2 and 81.7% of the variability in farrowing rates, respectively. After the combination of sperm traits, the relationship between percentage of LMNS with stable chromatin structure and farrowing rate was significant (r = 0.86, p < 0.05). The number of live piglets per parturition was not significantly correlated with sperm quality attributes. In conclusion, boar fertility after AI with freshly diluted semen can be predicted based on the evaluation of sperm morphology and chromatin integrity.
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