期刊
JOURNAL OF VETERINARY MEDICAL SCIENCE
卷 80, 期 10, 页码 1604-1609出版社
JAPAN SOC VET SCI
DOI: 10.1292/jvms.18-0121
关键词
bovine foamy virus; cattle; retrovirus; spumavirus; syncytium
资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan [S1491007]
Bovine foamy virus (BFV) is endemic in many countries, but has not been reported in Japan. A syncytium-forming virus was isolated from peripheral blood leukocytes of clinically healthy cattle on a farm in Kanagawa prefecture during a periodic epidemiological survey of viral diseases. The isolate was propagated in primary fetal bovine muscle cells and subsequently passaged in Madin-Darby bovine kidney cells. Since the isolate appeared to be distinct from the viruses with syncytium-forming ability previously isolated in Japan, we attempted to identify it using genomic analyses and electron microscopy. A phylogenetic analysis revealed that the isolate belongs to the bovine foamy virus cluster and is highly similar to a BFV strain isolated in China. A sero-epidemiological survey was performed using agar gel immunodiffusion test with the isolated virus as the antigen, and five of the 57 cattle tested were found to be seropositive.
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