期刊
JOURNAL OF VETERINARY MEDICAL SCIENCE
卷 73, 期 3, 页码 305-312出版社
JAPAN SOC VET SCI
DOI: 10.1292/jvms.10-0263
关键词
cattle; genotype-specific PCR; Japan; MPSP gene; Theileria orientalis
资金
- Japanese Ministry of Education, Science, Sports, Culture and Technology
- Japan Society for Promotion of Science (JSPS), Japan
- program for the Promotion of Basic Research Activities for Innovative Biosciences (PRO-BRAIN)
Theileria orientalis is a benign protozoan species that is widely distributed in Japan, yet sometimes causes serious economic losses in the livestock industry. In this study, we conducted a molecular survey based on genes encoding the major piroplasm surface protein (MPSP) and p23 for T. orientalis detected in cattle grazing in southern areas of Japan, consisting of 2 farms in Kumamoto prefecture (Aso and Kuma districts) and 3 farms in Okinawa prefecture (Ishigaki, Iriomote, and Yonaguni Islands). High prevalence rates of T. orientalis infection were shown in all the cattle populations using the diagnostic MPSP- and p23-PCR assays. Phylogenetic analyses revealed 4 MPSP genotypes and 3 p23 genotypes. Furthermore. MPSP genotype-specific PCR methods were developed in this study and wide distributions of 5-district genotypes of T. orientalis were observed for the examined farms. Our results indicate that at least 5 types of T. orientalis exist in Kumamoto and Okinawa prefectures of Japan and that genotype-specific PCR assays are highly applicable for the quarantine of transported cattle and for epidemiological surveys of bovine theileriosis in Japan.
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