期刊
JOURNAL OF VETERINARY MEDICAL SCIENCE
卷 73, 期 5, 页码 683-686出版社
JAPAN SOC VET SCI
DOI: 10.1292/jvms.10-0495
关键词
mouse Oas1b; SEAP reporter gene; subgenomic replicon; West Nile virus
资金
- 'Establishment of International Collaboration Centers for Zoonosis Control' of the Japan Society for the Promotion of Science
- Ministry of Education, Culture, Sports, Science and Technology [20405040]
- Ministry of Health, Labour and Welfare [H20-Shinkou-ippan-015]
- Grants-in-Aid for Scientific Research [20405040] Funding Source: KAKEN
We have developed a West Nile virus (WNV) subgenomic replicon harboring the secreted alkaline phosphatase (SEAP) reporter gene instead of viral structural genes (designated repWNV/SEAP). The repWNV/SEAP allowed easy evaluation of viral replication efficiency by direct measurement of SEAP secretion in the cell culture medium in physical containment level 2 facilities. Furthermore, we validated the availability of this system using a known anti-flavivirus gene, mouse oligoadenylate synthetase 1b (Oas 1b). The Oas1b-transfected cells were more resistant to repWNV/SEAP replication than the original cells. Thus, this system not only affords a useful tool for identification/evaluation of anti-flavivirus genes/drugs in terms of safety, ease of use and reliability, but should be able to reduce or replace the bioassay using laboratory animals.
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