期刊
JOURNAL OF VETERINARY MEDICAL SCIENCE
卷 72, 期 6, 页码 727-734出版社
JAPAN SOC VET SCI
DOI: 10.1292/jvms.09-0538
关键词
beta-lactamase; environmental samples; integron; multidrug resistance; public health
资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan
As little is known about antimicrobial resistance genes in fish farms, this study was conducted to monitor the incidence and prevalence of a wide range of antimicrobial resistance genes in Gram-negative bacteria isolated from water samples taken from fish farms in the northern part of Egypt. Ninety-one out of two hundred seventy-four (33.2%) non-repetitive isolates of Gram-negative bacteria showed multidrug resistance phenotypes and harbored at least one antimicrobial resistance gene. PCR and DNA sequencing results showed that 72 (26.3%) isolates contain tetracycline resistance genes and 19 (6.9%) isolates were positive for class 1 integrons with 12 different gene cassettes. The beta-lactamase-encoding genes were identified in 14(5.1%) isolates. The plasmid-mediated quinolone resistance genes, qnr and aac(6)-Ib-cr, were identified in 16(5.8%) and 3 (1.1%) isolates, respectively. Finally, the florphenicol resistance gene, floR, was identified in four (1.5%) isolates. To the best of our knowledge, this is the first report for molecular characterization of antimicrobial resistance in Gram-negative bacteria isolated from fish farms in Africa.
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