期刊
JOURNAL OF VETERINARY MEDICAL SCIENCE
卷 71, 期 8, 页码 1009-1015出版社
JAPAN SOC VET SCI
DOI: 10.1292/jvms.71.1009
关键词
Borna disease virus; epidemiology; raccoon
We have examined the seroprevalence of BDV in wild Raccoons (Procyon lotor) in Hokkaido, Japan. Serum samples from raccoons were examined using ELISA and Western blot assays to detect the presence of serum antibodies that react specifically to BDV antigens. Among 549 investigated individuals, eleven (2.0%) showed a positive reaction to BDV antigens. Brain tissue samples from five individuals were subjected to RT-PCR, which detected BDV sequences in three of them. Sequence analysis revealed a high degree of genetic conservation between BDV sequences derived from raccoons and previously published sequences derived from other animal species.
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