期刊
JOURNAL OF VETERINARY MEDICAL SCIENCE
卷 70, 期 12, 页码 1363-1366出版社
JAPAN SOC VET SCI
DOI: 10.1292/jvms.70.1363
关键词
arthropods; enzymes; molecular parasitology; tick; vector biology
资金
- Ministry of Education, Culture, Sports, Science, and Technology of Japan
- Basic Research Activities for Innovative Biosciences from the Bio-oriented Technology Research Advancement Institution
We previously identified two cDNAs from the midgut or adult Haemaphysalis longicornis that encode asparaginyl endopeptidases/legumains, HILgm and HILgm2. Functionally, both recombinant HILgm and HILgm2 efficiently digested blood proteins, haemoglobin and bovine serum albumin. Here, we investigated the expression profiles of legumain genes in the developmental stages in the life cycle of H. longicornis and in different tissues of adult ticks. Both HILgm and HILgm2 were well expressed in larvae, nymphs and adults. Legumain transcripts were expressed specifically in the midgut and were localized in some digestive vacuoles of gut epithelial cells. Furthermore, expression of either transcript was up-regulated by blood feeding in larvae and nymphs, Suggesting the important roles of legumains in blood feeding and blood-meal digestion in ticks.
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