期刊
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
卷 25, 期 2, 页码 203-209出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/1040638713476865
关键词
Fin biopsy; gill biopsy; nonlethal sampling; Viral hemorrhagic septicemia virus
资金
- New Visions Life Sciences Program at Cornell University
- Assistance Award to Cornell University [2007.883]
- Great Lakes Fishery Trust to the Western Fisheries Research Center
- United States Geological Survey, Seattle, WA
- project R/FTD-11 from the National Sea Grant College Program of the U.S. Department of Commerce's National Oceanic and Atmospheric Administration [NA10OAR4170064]
Nonlethal sampling is becoming a common method to diagnose fish diseases, especially with the availability of molecular testing. Viral hemorrhagic septicemia virus (VHSV) is a viral pathogen of finfish distributed worldwide. Although VHSV has been known to occur in some parts of the world for decades, a new genotype, IVb, recently emerged in the Laurentian Great Lakes of northeastern North America. Golden shiners (Notemigonus crysoleucas; Mitchill, 1814) and fathead minnows (Pimephales promelas; Rafinesque, 1820) were exposed to VHSV-IVb doses between 102 and 106 plaque forming units per fish by intraperitoneal injection at 10 degrees C. Both species experienced significant mortality after exposure, ranging from 38% to 52% in golden shiners and from 35% to 95% in fathead minnows. In golden shiners, a fin or gill sample was somewhat less sensitive at detecting VHSV-IVb by quantitative reverse transcription polymerase chain reaction (qRT-PCR) than a pooled organ sample (consisting of liver, anterior and posterior kidney, spleen, and heart), however the relative sensitivity increased when a fin and gill sample were tested in parallel. In fathead minnows, a fin or gill sample tested alone or in parallel was relatively more sensitive than a pooled organ sample by qRT-PCR. Specificity was 100% for all sample types in both species. The results suggest that fin and gill biopsies are useful tools to test for VHSV in live fish.
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