Wound healing and in vitro antioxidant activities of lipopeptides mixture produced by Bacillus mojavensis A21

The aim of the present study was to evaluate the in vitro antioxidant activities and the in vivo wound healing performance of lipopeptides produced by Bacillus mojavensis A21. The in vitro antioxidant activity was determined through four different assays: DPPH scavenging activity, reducing power, beta-carotene bleaching by linoleic acid assay and lipid peroxidation inhibition activity. The scavenging effect of A21 lipopeptides on 1, 1-dipheny1-2-picrylhydrazyl (DPPH) radical at 1 g/L was 65%. Moreover A21 lipopeptides showed good reducing power and significantly inhibited lipid peroxidation. The application of A21 lipopeptides gel on the wound site in a rat model accelerated significantly wound healing activity as compared to the control group, and a total closure was achieved after 13 days of wound induction. Further, histological study of biopsies treated with A21 lipopeptides showed fully re-epithelialized wound with a complete epidermal regeneration. (C) 2015 Elsevier Ltd. All rights reserved.