期刊
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
卷 24, 期 5, 页码 918-923出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/1040638712455171
关键词
Automated chemiluminescent assay; salivary cortisol; stress; porcine; validation
资金
- University of Murcia
- Spanish Ministry of Education and Science [AGL 2009-08509]
The aim of the current study was to validate an automated immunoassay for cortisol quantification in the saliva of pigs. The assay had intra- and interassay coefficients of variation lower than 16%, in all cases. The limit of detection was 0.016 mu g/dl, and the lower quantification limit was 0.197 mu g/dl. The assay also demonstrated a high level of accuracy, as determined by linearity under dilution and recovery tests. In addition, this assay was used to quantify cortisol in 2 models of stress: 1 in which animals were immobilized with a nose-snare and 1 in which pigs were transported for a duration of 30 min. In both cases, a significant increase (P < 0.01) in salivary cortisol was detected after the stressful situation. Overall, the assay validated in the present study could be used for the evaluation of cortisol changes in stressful situations.
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