期刊
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
卷 21, 期 5, 页码 649-654出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/104063870902100509
关键词
Fixation time; in situ hybridization; polymerase chain reaction; porcine circovirus; postweaning multisystemic wasting syndrome; probe size
资金
- Technology Development Program for Agriculture and Forestry
- Ministry for Agriculture, Forestry and Fisheries
- Korea Research Foundation [KRF-2006-005-J02902]
- National Research Foundation of Korea [2006-005-J02902] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Probe size and fixation time for detecting Porcine circovirus-2 (PCV-2) by in situ hybridization in formalin-fixed, paraffin-embedded lymph nodes from experimentally infected pigs were optimized. In situ hybridization using a 169-base pair (bp) probe detected significantly fewer PCV-2-positive cells than when using 8 other larger probes (P < 0.05). The difference in hybridization intensity between smaller probes (169 and 225 bp) and larger probes (416, 473, 571, 631, 693, and 753 bp) was statistically significant (P < 0.05). The PCV-2-positive cells were consistently detected in lymph nodes fixed Lip to 3 days; thereafter, the number of positive cells declined. The PCV-2-positive cells were detected in lymph nodes fixed for Lip to 730 days. The difference in hybridization intensity between samples fixed for a short term (1 or 3 days) and a longer term (4730 days) was statistically significant (P < 0.05). The data demonstrates that the optimal probe size and fixation time for detecting PCV-2 in formalin-fixed, paraffin-ern bedded lymph nodes is 473 bp and 1-3 days, respectively.
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