期刊
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
卷 20, 期 2, 页码 141-146出版社
AMER ASSOC VETERINARY LABORATORY DIAGNOSTICIANS INC
DOI: 10.1177/104063870802000201
关键词
Prion protein; scrapie; urine
资金
- Diachemix LLC. Milwaukee, Wisconsin, and the Canadian Food Inspection Agency [NBR 0421]
The misfolded form of cellular prion protein protein (PrP(C)) is the main component Of the infectious agent of transmissible spongiform encephalopathies and the validated biomarker for these diseases. The expression of PrP(C) is highest in the central nervous system and has been found in peripheral tissues. Soluble PrP(C) has been detected in cerebrospinal fluid, urine, serum, milk, and seminal plasma. In this study attempts were made to characterize prion protein ill urine S samples from normal and scrapie-infected sheep. Urine samples from scrapie-infected sheep and age-matched healthy sheep were collected and analyzed by Western blot following, concentration. A protease K-sensitive protein band with a molecular weight of approximately 27-30 kDa was visualized after immunoblotting with anti-PrP monoclonal antibodies to a C-terminal Part of PrP(C). but not after immunoblotting with monoclonal antibodies to ail N-terminal epitope of PrP(C) of with secondary antibodies only. The amount of PrP(C) in the urine of 49 animals (control group: n = 16; naturally scrapie-infected group): 11 = 33) was estimated by comparison with known amounts of ovine recombinant PrP in the immunoblot. Background concentration of PrP(C) in urine was found to be 0-0. 16 ng/ml (adjusted to the initial nonconcentrated volume of the urine samples). Seven out of 33 naturally scrapie-infected animals had ail elevated level (0.3-4.7 ng/ml) of PrP(C) in urine. The origin of PrP(C) in urine zinc the reason for the increased level of PrP(C) in scrapie-infected sheep urine has yet to be explored.
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