期刊
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
卷 20, 期 4, 页码 492-496出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/104063870802000413
关键词
Enterotoxigenic Escherichia coli; multiplex polymerase chain reaction; type 1 pili
资金
- Biogreen 21 [20070401034009]
- KRF [2006-005-J502901]
- Research Institute for Veterinary Science, Seoul National University
- Rural Development Administration (RDA), Republic of Korea [20070401034009] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Fimbriae and enterotoxins are major virulence factors associated with enterotoxigenic Escherichia coli (ETEC). In this study, 3 sets of multiplex polymerase chain reaction (mPCR) assays targeting fimbriae, enterotoxins, and other adherence factors were developed for detecting ETEC. A total number of 188 E coli Field isolates were examined, and percentages of E coli strains carrying each virulence factors were as follows: F4 (7.45%), F5 (29.79%), F6 (6.38%), F 18 (15.43%), F41 (3.72%), STa (10.11%), STb (20.74%), LT (9.57%), Stx2e (2.13%), EAST1 (42.02%), F1 (67.55%), AIDA-I (2.66%), and pAA (7.45%). Of the 188 E coli field isolates examined, 25.53% were found to be pathogenic ETEC, having both fimbriae and enterotoxins. However, the ratio increased to 44.68% when the presence of other adhesins was considered as criteria for virulence. Among the adherence factors, F1 was found to be the most prevalent. AIDA-I and pAA were also found with similar ratio as compared with other virulence factors. In addition, virulence patterns carrying these alternate adhesive genes with enterotoxins were detected with significant ratio. Therefore, it is desirable that alternate adhesins be considered as markers for diagnosis of ETEC.
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