期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 112, 期 27, 页码 8266-8271出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1506781112
关键词
metabolic engineering; microbial coculture; muconic acid; 4-hydroxybenzoic acid
资金
- US Department of Energy [DE-SC0008744]
- U.S. Department of Energy (DOE) [DE-SC0008744] Funding Source: U.S. Department of Energy (DOE)
Engineering microbial consortia to express complex biosynthetic pathways efficiently for the production of valuable compounds is a promising approach for metabolic engineering and synthetic biology. Here, we report the design, optimization, and scale-up of an Escherichia coli-E. coli coculture that successfully overcomes fundamental microbial production limitations, such as high-level intermediate secretion and low-efficiency sugar mixture utilization. For the production of the important chemical cis, cis-muconic acid, we show that the coculture approach achieves a production yield of 0.35 g/g from a glucose/xylose mixture, which is significantly higher than reported in previous reports. By efficiently producing another compound, 4-hydroxybenzoic acid, we also demonstrate that the approach is generally applicable for biosynthesis of other important industrial products.
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