期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 112, 期 42, 页码 12933-12937出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1511921112
关键词
integrated optics; optofluidics; single-virus detection; biosensing; multimode interferometer
资金
- W. M. Keck Center for Nanoscale Optofluidics at University of California, Santa Cruz
- NIH [4R33AI100229, 1R21AI100229]
- National Science Foundation (NSF) [CBET-1159453, CBET-1159423]
- Eugene Cota-Robles Fellowship
- NSF [DGE 0809125]
Optical waveguides simultaneously transport light at different colors, forming the basis of fiber-optic telecommunication networks that shuttle data in dozens of spectrally separated channels. Here, we re-imagine this wavelength division multiplexing (WDM) paradigm in a novel context-the differentiated detection and identification of single influenza viruses on a chip. We use a single multimode interference (MMI) waveguide to create wavelength-dependent spot patterns across the entire visible spectrum and enable multiplexed single bio-molecule detection on an optofluidic chip. Each target is identified by its time-dependent fluorescence signal without the need for spectral demultiplexing upon detection. We demonstrate detection of individual fluorescently labeled virus particles of three influenza A subtypes in two implementations: labeling of each virus using three different colors and two-color combinatorial labeling. By extending combinatorial multiplexing to three or more colors, MMI-based WDM provides the multiplexing power required for differentiated clinical tests and the growing field of personalized medicine.
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