期刊
JOURNAL OF VACUUM SCIENCE & TECHNOLOGY A
卷 29, 期 1, 页码 -出版社
A V S AMER INST PHYSICS
DOI: 10.1116/1.3531693
关键词
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资金
- Defense Advanced Research Projects Agency (DARPA) [N66001-09-1-2103]
- UROP at the University of Minnesota
- NSF
Separating long DNA in a microfabricated post array requires (tens of) thousands of posts in the separation channel. Moving from microposts to nanoposts is thus a fabrication challenge owing to the large area that needs to be nanopatterned. The authors implemented an oxygen plasma etching method in conjunction with conventional optical photolithography and deep trench etching that led to centimeter-long microchannels containing either 360 or 460 nm diameter posts in a hexagonal array with a 3 mu m spacing. Separations of the XhoI lambda-DNA digest in the device indicate that these sparse nanopost arrays are an improvement over the equivalent micropost array with only a marginal increase in fabrication cost. The fabrication method described here is broadly applicable to biological microfluidic and nanofluidic platforms that require nanoscale features with micrometer-scale spacing. (C) 2011 American Vacuum Society [DOI: 10.1116/1.3531693]
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