期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 112, 期 19, 页码 6164-6169出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1422340112
关键词
Cas9; CRISPR-Cas; hepatitis C virus; RNA targeting; Francisella
资金
- National Institutes of Health (NIH) [R01-AI11070, U54-AI057157]
- Burroughs Wellcome Fund
- Yerkes Research Center Base Grant [P51RR-000165]
- NIH [R01-AI070101, R01-DK083356]
- National Science Foundation Graduate Research Fellowship Program
- Achievement Rewards for College Scientists Foundation
Clustered, regularly interspaced, short palindromic repeats-CRISPR associated (CRISPR-Cas) systems are prokaryotic RNA-directed endonuclease machineries that act as an adaptive immune system against foreign genetic elements. Using small CRISPR RNAs that provide specificity, Cas proteins recognize and degrade nucleic acids. Our previous work demonstrated that the Cas9 endonuclease from Francisella novicida (FnCas9) is capable of targeting endogenous bacterial RNA. Here, we show that FnCas9 can be directed by an engineered RNA-targeting guide RNA to target and inhibit a human +ssRNA virus, hepatitis C virus, within eukaryotic cells. This work reveals a versatile and portable RNA-targeting system that can effectively function in eukaryotic cells and be programmed as an antiviral defense.
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