期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 112, 期 23, 页码 7225-7230出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1508224112
关键词
regulatory T cells; FOXP3; CD15s; autoimmunity; tumor immunity
资金
- Assistance Publique - Hopitaux de Paris (Programme Hospitalier de Recherche Clinique) [AOR09-085]
- Centre d'Investigation Biologique Pitie-Salpetriere, Societe Nationale Francaise de Medecine Interne
- Fondation Arthritis
- Ministry of Education, Culture, Sports, Science and Technology of Japan [20002007, 26253030]
- Japan Science and Technology Agency
- Grants-in-Aid for Scientific Research [15K08288, 26253030, 20002007] Funding Source: KAKEN
CD4(+) regulatory T (Treg) cells expressing CD25 and the transcription factor forkhead box P3 (FOXP3) are indispensable for immunological self-tolerance and homeostasis. FOXP3(+) CD25(+) CD4(+) T cells in humans, however, are heterogeneous in function and differentiation status, including suppressive or nonsuppressive cells as well as resting or activated Treg cells. We have searched for cell surface markers specific for suppression-competent Treg cells by using a panel of currently available monoclonal antibodies reactive with human T cells. We found that CD15s (sialyl Lewis x) was highly specific for activated, terminally differentiated, and most suppressive FOXP3(high) effector Treg (eTreg) cells and able to differentiate them in various clinical settings from nonsuppressive FOXP3(+) T cells secreting inflammatory cytokines. For example, CD15s(+) FOXP3(+) eTreg cells were increased in sarcoidosis, whereas it was nonsuppressive CD15s(-) FOXP3(+) T cells that were expanded in lupus flares. FOXP3(+) cells induced from conventional CD4(+) T cells by T-cell receptor stimulation hardly expressed CD15s. CD15s(+) CD4(+) T-cell depletion was sufficient to evoke and enhance in vitro immune responses against tumor or viral antigens. Collectively, we have identified CD15s as a biomarker instrumental in both phenotypic and functional analysis of FOXP3(+) CD4(+) T-cell subpopulations in health and disease. It allows specific targeting of eTreg cells, rather than whole FOXP3(+) CD4(+) T cells, in controlling immune responses.
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