期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 112, 期 29, 页码 E3800-E3805出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1500925112
关键词
organometallic anticancer compound; cancer metabolism; mitochondria; transcriptome sequencing; protein microarrays
资金
- Biotechnology and Biological Sciences Research Council [324594]
- European Research Council [324594, 247450]
- Wellcome Trust [086357, 102696]
- European Union COST Action [CM1105]
- Biotechnology and Biological Sciences Research Council [990261] Funding Source: researchfish
- European Research Council (ERC) [324594] Funding Source: European Research Council (ERC)
The organometallic half-sandwich compound [Os(eta(6)-p-cymene) (4-(2-pyridylazo)-N, N-dimethylaniline) I] PF6 is 49x more potent than the clinical drug cisplatin in the 809 cancer cell lines that we screened and is a candidate drug for cancer therapy. We investigate the mechanism of action of compound 1 in A2780 epithelial ovarian cancer cells. Whole-transcriptome sequencing identified three missense mutations in the mitochondrial genome of this cell line, coding for ND5, a subunit of complex I (NADH dehydrogenase) in the electron transport chain. ND5 is a proton pump, helping to maintain the coupling gradient in mitochondria. The identified mutations correspond to known protein variants (p. I257V, p. N447S, and p. L517P), not reported previously in epithelial ovarian cancer. Time-series RNA sequencing suggested that osmium-exposed A2780 cells undergo a metabolic shunt from glycolysis to oxidative phosphorylation, where defective machinery, associated with mutations in complex I, could enhance activity. Downstream events, measured by time-series reverse-phase protein microarrays, high-content imaging, and flow cytometry, showed a dramatic increase in mitochondrially produced reactive oxygen species (ROS) and subsequent DNA damage with upregulation of ATM, p53, and p21 proteins. In contrast to platinum drugs, exposure to this organo-osmium compound does not cause significant apoptosis within a 72-h period, highlighting a different mechanism of action. Superoxide production in ovarian, lung, colon, breast, and prostate cancer cells exposed to three other structurally related organo-Os(II) compounds correlated with their antiproliferative activity. DNA damage caused indirectly, through selective ROS generation, may provide a more targeted approach to cancer therapy and a concept for next-generation metal-based anticancer drugs that combat platinum resistance.
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