期刊
JOURNAL OF UROLOGY
卷 188, 期 4, 页码 1361-1368出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.juro.2012.06.004
关键词
prostate; prostatic neoplasms; autophagy; receptors, androgen; 1,7-bis(4-hydroxy-3-methoxyphenyl)-1,4,6-heptatrien-3-one
资金
- National Institutes of Health [CA156700]
- George Whipple Professorship Endowment
- Taiwan Department of Health Clinical Trial and Research Center of Excellence [DOH99-TD-B-111-004]
Purpose: Androgen receptor has a critical role in prostate cancer development and progression. Cell death via autophagy may also contribute to prostate cancer progression. We determined the role and regulatory effects of androgen receptor on the autophagy process of prostate cancer cells. Materials and Methods: Using a series of morphological approaches, such as transmission electron microscopy, monodansylcadaverine (Sigma (R)) and GFP-LC3 fluorescence microscopy assay, and Western blot we monitored the autophagic process in 3 pairs of prostate cancer cell lines to study the relationship to androgen receptor signals. Results: Androgen receptor knockdown in androgen receptor positive cells, such as LNCaP or CWRrv1 human prostate cancer cells, led to increased autophagy. Adding functional androgen receptor to androgen receptor negative cells, such as PC3 human prostate cancer cells, resulted in decreased autophagy. This suggests that androgen receptor could have a negative role in regulating autophagy. Mechanism dissection indicated that androgen receptor might repress autophagy via modulation of p62 expression. A therapeutic approach of targeting androgen receptor to increase autophagy using the androgen receptor degradation enhancer ASC-J9 (R) suppressed prostate cancer growth. Conclusions: Findings provide evidence that the androgen receptor might promote prostate cancer cell growth via autophagy down-regulation. Targeting the androgen receptor via ASC-J9 might lead to tumor suppression via the induction of autophagy. This may represent a new, potential therapeutic approach and mechanism for prostate cancer.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据