期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 112, 期 8, 页码 2593-2598出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1419921112
关键词
intraocular pressure; glaucoma; ganglion cells; bipolar cells; AII amacrine cells
资金
- NIH [EY004446, EY019908, EY021479]
- Retina Research Foundation (Houston)
- Research to Prevent Blindness
- NIH Vision Core [EY002520]
Glaucoma is the second leading cause of blindness in the United States and the world, characterized by progressive degeneration of the optic nerve and retinal ganglion cells (RGCs). Glaucoma patients exhibit an early diffuse loss of retinal sensitivity followed by focal loss of RGCs in sectored patterns. Recent evidence has suggested that this early sensitivity loss may be associated with dysfunctions in the inner retina, but detailed cellular and synaptic mechanisms underlying such sensitivity changes are largely unknown. In this study, we use whole-cell voltage-clamp techniques to analyze light responses of individual bipolar cells (BCs), AII amacrine cells (AIIACs), and ON and sustained OFF alpha-ganglion cells (ON alpha GCs and sOFF alpha GCs) in dark-adapted mouse retinas with elevated intraocular pressure (IOP). We present evidence showing that elevated IOP suppresses the rod ON BC inputs to AIIACs, resulting in less sensitive AIIACs, which alter AIIAC inputs to ON alpha GCs via the AIIAC-->cone ON BC-->ON alpha GC pathway, resulting in lower ON alpha GC sensitivity. The altered AIIAC response also reduces sOFF alpha GC sensitivity via the AIIAC-->sOFF alpha GC chemical synapses. These sensitivity decreases in alpha GCs and AIIACs were found in mice with elevated IOP for 3-7 wk, a stage when little RGC or optic nerve degeneration was observed. Our finding that elevated IOP alters neuronal function in the inner retina before irreversible structural damage occurs provides useful information for developing new diagnostic tools and treatments for glaucoma in human patients.
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