期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 112, 期 8, 页码 2425-2430出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1411514112
关键词
SHAPE; conformational change; in vivo; ribosome; 16S rRNA
资金
- National Science Foundation [MCB-1121024, MCB-1243997]
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [1121024, 1243997] Funding Source: National Science Foundation
It was shown decades ago that purified 30S ribosome subunits readily interconvert between active and inactive conformations in a switch that involves changes in the functionally important neck and decoding regions. However, the physiological significance of this conformational change had remained unknown. In exponentially growing Escherichia coli cells, RNA SHAPE probing revealed that 16S rRNA largely adopts the inactive conformation in stably assembled, mature 30S subunits and the active conformation in translating (70S) ribosomes. Inactive 30S subunits bind mRNA as efficiently as active subunits but initiate translation more slowly. Mutations that inhibited interconversion between states compromised translation in vivo. Binding by the small antibiotic paromomycin induced the inactive-to-active conversion, consistent with a low-energy barrier between the two states. Despite the small energetic barrier between states, but consistent with slow translation initiation and a functional role in vivo, interconversion involved large-scale changes in structure in the neck region that likely propagate across the 30S body via helix 44. These findings suggest the inactive state is a biologically relevant alternate conformation that regulates ribosome function as a conformational switch.
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