期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 112, 期 37, 页码 E5117-E5122出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1509663112
关键词
micromechanics; collagen; fiber network
资金
- Office of Naval Research via Oregon Nanoscience and Microtechnologies Institute Nanometrology and Nanoelectronics Initiative [N00014-07-1-0457]
- National Science Foundation [PHY-1400968]
- National Science Foundation Center for Theoretical Biological Physics [PHY-1308264]
- Cancer Prevention and Research Institute of Texas Scholar Program of the State of Texas at Rice University
- Division Of Computer and Network Systems
- Direct For Computer & Info Scie & Enginr [1338099] Funding Source: National Science Foundation
- Division Of Physics
- Direct For Mathematical & Physical Scien [1400968] Funding Source: National Science Foundation
Collagen gels are widely used in experiments on cell mechanics because they mimic the extracellular matrix in physiological conditions. Collagen gels are often characterized by their bulk rheology; however, variations in the collagen fiber microstructure and cell adhesion forces cause the mechanical properties to be inhomogeneous at the cellular scale. We study the mechanics of type I collagen on the scale of tens to hundreds of microns by using holographic optical tweezers to apply pN forces to micro-particles embedded in the collagen fiber network. We find that in response to optical forces, particle displacements are inhomogeneous, anisotropic, and asymmetric. Gels prepared at 21 degrees C and 37 degrees C show qualitative difference in their micromechanical characteristics. We also demonstrate that contracting cells remodel the micromechanics of their surrounding extracellular matrix in a strain- and distance-dependent manner. To further understand the micromechanics of cellularized extracellular matrix, we have constructed a computational model which reproduces the main experiment findings.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据