期刊
JOURNAL OF TRANSLATIONAL MEDICINE
卷 12, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s12967-014-0304-0
关键词
Regulatory T cells; Regulatory B cells; Lung cancer; Lymphocytes; Microenvironment
资金
- Shanghai Leading Academic Discipline Project [B115]
- Zhongshan Distinguished Professor Grant (XDW)
- National Nature Science Foundation of China [91230204, 81270099, 81320108001, 81270131]
- Shanghai Committee of Science and Technology [12JC1402200, 12431900207, 11410708600, 14431905100]
- Zhejiang Provincial Natural Science Foundation [Z2080988]
- Zhejiang Provincial Science Technology Department Foundation [2010C14011]
- Ministry of Education
- Academic Special Science and Research Foundation for PhD Education [201300/1110043]
Background: Regulatory T cells (Tregs) and B cells (Bregs) play an important role in the development of lung cancer. The present study aimed to investigate the phenotype of circulating Tregs and Bregs in patients with lung cancer and explore potential mechanism by which lung cancer cells act on the development of both. Methods: Patients with lung cancer (n = 268) and healthy donors (n = 65) were enrolled in the study. Frequencies of Tregs and Bregs were measured by flow cytometry with antibodies against CD4, CD25, CD127, CD45RA, CD19, CD24, CD27 and IL-10 before and after co-cultures. qRT-PCR was performed to evaluate the mRNA levels of RANTES, MIP-1 alpha, TGF-beta, IFN-gamma and IL-4. Results: We found a lower frequency of Tregs and a higher frequency of Bregs in patients with lung cancer compared to healthy donors. Co-culture of lung cancer cells with peripheral blood mononuclear cells could polarize the lymphocyte phenotype in the similar pattern. Lipopolysaccharide (LPS)-stimulated lung cancer cells significantly modulated regulatory cell number and function in an in vitro model. Conclusion: We provide initial evidence that frequencies of peripheral Tregs decreased or Bregs increased in patients with lung cancer, which may be modulated directly by lung cancer cells. It seems cancer cells per se plays a crucial role in the development of tumor immunity.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据