Bfr1p is responsible for tributyltin resistance in Schizosaccharomyces pombe

ATP-binding cassette (ABC) transporter plays an important role for resistance against xenobiotics. There are eleven ABC transporter genes in the genome of fission yeast Schizosaccharomyces pombe. We examined the role of ABC transporter against the toxicity of tributyltin chloride (TBT), a widespread environmental pollutant, in cell growth. Among individual ABC transporter mutants, the growth of a mutant deficient in Bfr1p, a plasma membrane-embedded transporter, was extremely sensitive to TBT. The lethal TBT concentration inducing 50% of cell death (LC50) was 25 mu M for the parent strain and 10.2 mu m for the bfr1 Delta mutant. Thus, Bfr1p was responsible for TBT resistance in S. pombe.