期刊
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
卷 9, 期 11, 页码 E13-E26出版社
WILEY
DOI: 10.1002/term.1736
关键词
mesenchymal stem cells; hydrogel biomaterials; 6-bromoindirubin-3 '-oxime; proliferation; pluripotency; regenerative medicine
类别
资金
- Orthopaedic Research and Education Foundation/Musculoskeletal Transplant Foundation (OREF/MTF)
- Rochester/Finger Lakes Eye and Tissue Bank (RETB/FLETB)
- National Institutes of Health (NIH
- T32, Training in Orthopaedic Research) [NIH-T32AR053459]
Promoting mesenchymal stem cell (MSC) proliferation has numerous applications in stem cell therapies, particularly in the area of regenerative medicine. In order for cell-based regenerative approaches to be realized, MSC proliferation must be achieved in a controlled manner without compromising stem cell differentiation capacities. Here we demonstrate that 6-bromoindirubin-3'-oxime (BIO) increases MSC beta-catenin activity 106-fold and stem cell-associated gene expression similar to 33-fold, respectively, over untreated controls. Subsequently, BIO treatment increases MSC populations 1.8-fold in typical 2D culture conditions, as well as 1.3-fold when encapsulated within hydrogels compared to untreated cells. Furthermore, we demonstrate that BIO treatment does not reduce MSC multipotency where MSCs maintain their ability to differentiate into osteoblasts, chondrocytes and adipocytes using standard conditions. Taken together, our results demonstrate BIO's potential utility as a proliferative agent for cell transplantation and tissue regeneration. Copyright (C) 2013 John Wiley & Sons, Ltd.
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