期刊
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
卷 8, 期 3, 页码 186-194出版社
WILEY
DOI: 10.1002/term.1512
关键词
oligodendrocytes; PuraMatrix; neural tissue engineering; hydrogel; encapsulation; astrocytes; neuronal network activity
类别
资金
- Academy of Finland [123233]
- Tampere University Hospital
- Finnish Cultural Foundation/ Pirkanmaa Regional Fund
- Finnish Funding Agency for Technology and Innovation (StemInClin-project)
- Academy of Finland (AKA) [123233, 123233] Funding Source: Academy of Finland (AKA)
The future of tissue engineering applications for neuronal cells will require a supportive 3D matrix. This particular matrix should be soft, elastic and supportive for cell growth. In this study, we characterized the suitability of a 3D synthetic hydrogel matrix, PuraMatrix (TM), as a growth platform for human embryonic stem cell (hESC)-derived neural cells. The viability of the cells grown on top of, inside and under the hydrogel was monitored. The maturation and electrical activity of the neuronal networks inside the hydrogel were further characterized. We showed that cells stayed viable on the top of the PuraMatrix (TM) surface and growth of the neural cells and neural processes was good. Further, hESC-derived neurons, astrocytes and oligodendrocytes all grew, matured and migrated when cultured inside the hydrogel. Importantly, neuronal cells were able to form electrically active connections that were verified using microelectrode array. Thus, PuraMatrix is a good supportive growth matrix for human neural cells and may serve as a matrix for neuronal scaffolds in neural tissue engineering. Copyright (c) 2012 John Wiley & Sons, Ltd.
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