期刊
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
卷 9, 期 10, 页码 1161-1171出版社
WILEY-BLACKWELL
DOI: 10.1002/term.1664
关键词
adipose-derived stem cells; bone healing; electromagnetic field; osteogenic differentiation; pretreatment; scaffold
类别
资金
- National Research Foundation of Korea (NRF) - Korean government (MEST) [2012-0001235]
- World Class University (WCU) programme through the National Research Foundation of Korea - Ministry of Education, Science and Technology [R31-2008-000-10105-0]
- National Research Foundation of Korea [21A20131700001] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
An electromagnetic field is an effective stimulation tool because it promotes bone defect healing, albeit in an unknown way. Although electromagnetic fields are used for treatment after surgery, many patients prefer cell-based tissue regeneration procedures that do not require daily treatments. This study addressed the effects of an electromagnetic field on adipose-derived stem cells (ASCs) to investigate the feasibility of pretreatment to accelerate bone regeneration. After identifying a uniform electromagnetic field inside a solenoid coil, we observed that a 45Hz electromagnetic field induced osteogenic marker expression via bone morphogenetic protein, transforming growth factor , and Wnt signalling pathways based on microarray analyses. This electromagnetic field increased osteogenic gene expression, alkaline phosphate activity and nodule formation in vitro within 2weeks, indicating that this pretreatment may provide osteogenic potential to ASCs on three-dimensional (3D) ceramic scaffolds. This pretreatment effect of an electromagnetic field resulted in significantly better bone regeneration in a mouse calvarial defect model over 4weeks compared to that in the untreated group. This short-term evaluation showed that the electromagnetic field pretreatment may be a future therapeutic option for bone defect treatment. Copyright (c) 2012 John Wiley & Sons, Ltd.
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