期刊
JOURNAL OF THERMAL BIOLOGY
卷 46, 期 -, 页码 31-39出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jtherbio.2014.10.001
关键词
Acute heat stress; House sparrow; Primary fibroblasts; Muscle fiber; Metabolic rates
资金
- NSF [IBN 0212587]
- Division Of Integrative Organismal Systems
- Direct For Biological Sciences [1036914] Funding Source: National Science Foundation
Given that our climate is rapidly changing, Physiological Ecologists have the critical task of identifying characteristics of species that make them either resilient or susceptible to changes in their natural air temperature regime. Because climate change models suggest that heat events will become more common, and in some places more extreme, it is important to consider how extreme heat events might affect the physiology of a species. The implications of more frequent heat wave events for birds have only recently begun to be addressed, however, the impact of these events on the cellular physiology of a species is difficult to assess. We have developed a novel approach using dermal fibroblasts to explore how short-term thermal stress at the whole animal level might affect cellular rates of metabolism. House sparrows, Passer domesticus were separated into a control group and a heat shocked group, the latter acclimated to 43 degrees C for 24 h. We determined the plasticity of cellular thermal responses by assigning a recovery group that was heat shocked as above, but then returned to room temperature for 24 h. Primary dermal fibroblasts were grown from skin of all treatment groups and the pectoralis muscle was collected. We found that glycolysis (ECAR) and oxygen consumption rates (OCR), measured using a Seahorse XF 96 analyzer, were significantly higher in the fibroblasts from the heat shocked group of House sparrows compared with their control counterparts. Additionally, muscle fiber diameters decreased and, in turn, Na+ -K+-ATPase maximal activity in the muscle significantly increased in heat shocked sparrows compared with birds in the control group. All of these physiological alterations due to short-term heat exposure were reversible within 24 h of recovery at room temperature. These results show that acute exposure to heat stress significantly alters the cellular physiology of sparrows, but that this species is plastic enough to recover from such a thermal insult within 24 h. (C) 2014 Published by Elsevier Ltd.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据