期刊
JOURNAL OF THEORETICAL BIOLOGY
卷 266, 期 4, 页码 550-559出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jtbi.2010.07.006
关键词
Enzyme activities ratio; Pure birth process with killing; Restriction endonuclease; Methyltransferase
资金
- Russian Foundation for Basic Research [09-04-01098]
- Howard Hughes Medical Institute [55005610]
- NIH [GM9295]
- Russian Academy of Sciences
- Russian Science Agency [2.740.11.0101]
The success of a phage that infects a bacterial cell possessing a restriction-modification (R-M) system depends on the activities of the host methyltransferase and restriction endonuclease, and the number of susceptible sites in the phage genome. However, there is no model describing this dependency and linking it to observable parameters such as the fraction of surviving cells under excess phage, or probability of plating at low amount of phages. We model the phage infection of a cell with a R-M system as a pure birth process with a killing state. We calculate the transitional probabilities and the stationary distribution for this process. We generalize the model developed for a single cell to the case of multiple identical cells invaded by a Poisson-distributed number of phages. The R-M enzyme activities are assumed to be constant, time-dependent, or random. The obtained results are used to estimate the ratio of the methyltransferase and endonuclease activities from the observed fraction of surviving cells. (c) 2010 Elsevier Ltd. All rights reserved.
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