期刊
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
卷 90, 期 4, 页码 599-607出版社
WILEY
DOI: 10.1002/jsfa.3856
关键词
red kidney bean (Phaseolus vulgaris L.); phaseolin; protein isolate; acid-extraction technique; functional property; conformation
资金
- National Natural Science Foundation of China [20876057, 30972049]
- Doctorate Foundation of South China University of Technology
BACKGROUND: Kidney bean (Phaseolus vulgris L.) seed is an underutilised plant protein source with good potential to be applied in the food industry. Phaseolin (also named G1 globulin) represents about 50 g kg(-1) of total storage protein in the seed. The aim of the present study was to characterise physicochemical, functional and conformational properties of phaseolin, and to compare these properties with those of kidney bean protein isolate (KPI). RESULTS: Compared with kidney bean protein isolate (KPI), the acid-extracted phaseolin-rich protein product (PRP) had much lower protein recovery of 320 9 kg(-1) (dry weight basis) but higher phaseolin purity (over 950 g kg(-1)). PRP contained much lower sulfhydryl (SH) and disulfide bond contents than KPI. Differential scanning calorimetry analyses showed that the phaseolin in PRP was less denatured than in KPI. Thermal analyses in the presence or absence of dithiothreitol, in combination with SH and SS content analyses showed the contributions of SS to the thermal stability of KPI. The analyses of near-UV circular dichroism and intrinsic fluorescence spectra indicated more compacted tertiary conformation of the proteins in PRP than in KPI. PRP exhibited much better protein solubility, emulsifying activity index, and gel-forming ability than KPI. The relatively poor functional properties of KPI may be associated with protein denaturation/unfolding, with subsequent protein aggregation. CONCLUSION: The results presented here suggest the potential for acid-extracted PRP to be applied in food formulations, in view of its functional properties. (C) 2009 Society of Chemical Industry
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