期刊
JOURNAL OF THE ROYAL SOCIETY INTERFACE
卷 11, 期 98, 页码 -出版社
ROYAL SOC
DOI: 10.1098/rsif.2014.0501
关键词
cell culture; biocompatibility; collagen; tissue engineering
资金
- Institute of Making, University College London Summer Studentship
- BBSRC David Phillips
- BBSRC [BB/F023774/1] Funding Source: UKRI
- EPSRC [EP/I00159X/2, EP/I00159X/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/F023774/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/I00159X/1, EP/I00159X/2] Funding Source: researchfish
A challenge in three-dimensional tissue culture remains the lack of quantitative information linking nutrient delivery and cellular distribution. Both in vivo and in vitro, oxygen is delivered by diffusion from its source (blood vessel or the construct margins). The oxygen level at a defined distance from its source depends critically on the balance of diffusion and cellular metabolism. Cells may respond to this oxygen environment through proliferation, death and chemotaxis, resulting in spatially resolved gradients in cellular density. This study extracts novel spatially resolved and simultaneous data on tissue oxygenation, cellular proliferation, viability and chemotaxis in three-dimensional spiralled, cellular collagen constructs. Oxygen concentration gradients drove preferential cellular proliferation rates and viability in the higher oxygen zones and induced chemotaxis along the spiral of the collagen construct; an oxygen gradient of 1.03 mmHg mm(-1) in the spiral direction induced a mean migratory speed of 1015 mu mday(-1). Although this movement was modest, it was effective in balancing the system to a stable cell density distribution, and provided insights into the natural cell mechanism for adapting cell number and activity to a prevailing oxygen regime.
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