4.6 Article

Adhesion formation of primary human osteoblasts and the functional response of mesenchymal stem cells to 330 nm deep microgrooves

期刊

JOURNAL OF THE ROYAL SOCIETY INTERFACE
卷 5, 期 27, 页码 1231-1242

出版社

ROYAL SOC
DOI: 10.1098/rsif.2008.0035

关键词

osteoblasts; mesenchymal stem cells; focal adhesions; genomic regulation; nanotopography; nanobioscience

资金

  1. Biotechnology and Biological Sciences Research Council [JF20604] Funding Source: Medline
  2. Wellcome Trust Funding Source: Medline

向作者/读者索取更多资源

The surface microtexture of an orthopaedic device can regulate cellular adhesion, a process fundamental in the initiation of osteoinduction and osteogenesis. Advances in fabrication techniques have evolved to include the field of surface modification; in particular, nanotechnology has allowed for the development of experimental nanoscale substrates for investigation into cell nanofeature interactions. Here primary human osteoblasts (HOBs) were cultured on ordered nanoscale groove/ridge arrays fabricated by photolithography. Grooves were 330 nm deep and either 10, 25 or 100 mu m in width. Adhesion subtypes in HOBs were quantified by immunofluorescent microscopy and cell substrate interactions were investigated via immunocytochemistry with scanning electron microscopy. To further investigate the effects of these substrates on cellular function, 1.7 K gene microarray analysis was used to establish gene regulation profiles of mesenchymal stem cells cultured on these nanotopographies. Nanotopographies significantly affected the formation of focal complexes (FXs), focal adhesions (FAs) and supermature adhesions (SMAs). Planar control substrates induced widespread adhesion formation; 100 mm wide groove/ridge arrays did not significantly affect adhesion formation yet induced upregulation of genes involved in skeletal development and increased osteospecific function; 25 mu m wide groove/ridge arrays were associated with a reduction in SMA and an increase in FX formation; and 10 mu m wide groove/ridge arrays significantly reduced osteoblast adhesion and induced an interplay of up- and downregulation of gene expression. This study indicates that groove/ridge topographies are important modulators of both cellular adhesion and osteospecific function and, critically, that groove/ridge width is important in determining cellular response.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据