期刊
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION
卷 30, 期 10, 页码 1980-1987出版社
OPTICAL SOC AMER
DOI: 10.1364/JOSAA.30.001980
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资金
- University of Georgia Research Foundation
- National Science Foundation [MCB1052672]
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1052672] Funding Source: National Science Foundation
Phase diversity (PD) is a powerful technique for estimating wavefront aberrations from two-dimensional images of extended scenes. PD can work with extended incoherent images and, in an adaptive optics system, does not need extra hardware in addition to the deformable mirror. For these reasons, PD should be well suited to aberration measurement in microscopy applications. But, in biological widefield microscopy, the objects being imaged are frequently three-dimensional, and the images contain out-of-focus light. In this paper, we introduce multiplane PD and show that PD can be extended to widefield imaging of three-dimensional objects. This should be particularly useful in the field of biological fluorescence microscopy where the objects are very light sensitive and the aberrations cannot easily be determined. (C) 2013 Optical Society of America
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