期刊
JOURNAL OF THE NORTH AMERICAN BENTHOLOGICAL SOCIETY
卷 29, 期 3, 页码 1058-1074出版社
NORTH AMER BENTHOLOGICAL SOC
DOI: 10.1899/09-108.1
关键词
mtDNA; integrative taxonomy; cryptic species; DNA taxonomy; larval association
资金
- German Academy of Sciences [BMBF-LPD 9901/8-169]
- Trichoptera Barcode of Life
- Natural Sciences and Engineering Research Council (NSERC)
- Genome Canada
- Canadian Centre for DNA Barcoding, University of Guelph
- Minnesota Agricultural Experiment Station [AES 0017015, AES 0017017]
- National Science Foundation [DEB 0117772]
Mitochondrial deoxyribonucleic acid (mtDNA) sequence data have been both heralded and scrutinized for their ability or lack thereof to discriminate among species for identification (DNA barcoding) or description (DNA taxonomy). Few studies have systematically examined the ability of mtDNA from the DNA barcode region (658 base pair fragment of the 5' terminus of the mitochondrial cytochrome c oxidase I gene) to distinguish species based on range-wide sampling of specimens from closely related species. Here we examined the utility of DNA barcode data for delimiting species, associating life stages, and as a potential genetic marker for phylogeographic studies by analyzing a range-wide sample of closely related Chilean representatives of the caddisfly genus Smicridea subgenus Smicridea. Our data revealed the existence of 7 deeply diverged, previously unrecognized lineages and confirmed the existence of 2 new species: Smicridea (S.) patinae, new species and Smicridea (S.) lourditae, new species. Based on our current taxonomic evaluation, we considered the other 5 lineages to be cryptic species. The DNA barcode data proved useful in delimiting species within Chilean Smicridea (Smicridea) and were suitable for life-stage associations. The data also contained sufficient intraspecific variation to make the DNA barcode a candidate locus for widespread application in phylogeographic studies.
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