期刊
POULTRY SCIENCE
卷 94, 期 5, 页码 947-954出版社
OXFORD UNIV PRESS
DOI: 10.3382/ps/pev086
关键词
chicken astrovirus; chicken embryonated eggs; viral isolation; enteric virus
资金
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico fellowship
Astroviruses have been associated with enteric disorders in many animal species, including chickens. Here, we describe the isolation, propagation, and pathological characteristics of chicken astrovirus (CAstV) in specific pathogen free (SPF) chicken embryonated eggs (CEE) from chickens with diarrhea and runting-stunting syndrome. The CEE were inoculated via the yolk sac route. Viral confirmation was carried out using PCR techniques and transmission electron microscopy negative staining with ammonium molybdate. The intestinal contents were screened for CAstV, and differential diagnostic testing was performed for avian nephritis virus, avian rotavirus, avian reovirus, chicken parvovirus, infectious bronchitis virus, and fowl adenovirus Group I to detect co-infection with other infectious agents. Seven-or 14-day-old CEEs presented with hemorrhages, edema, a gelatinous aspect, deformities, and dwarfism. The supporting membranes did not show any alterations. Here, we have described the isolation of CAstV and its pathological characteristics in SPF CEE.
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