期刊
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
卷 27, 期 -, 页码 64-76出版社
ELSEVIER
DOI: 10.1016/j.jmbbm.2013.06.009
关键词
Reverse thermo-responsive; Biphasic calcium phosphates; Bioceramics; RhBMP-2 carriers; Non-union defect
资金
- Ministry of Economic Affairs [100-EC-17-A-19-S1-176]
- National Science Council in Taiwan [NSC99-2628-B-037-002-MY3, NSC 100-2628-E-037-002]
- Kaohsiung Medical University Hospital [KMUH99-9R36, KMUH100-0R40]
Porous biphasic calcium phosphates (BCP) were fabricated using reverse thermoresponsive hydrogels with hydroxyapatite (HAp) and beta-tricalcium (beta-TCP) powder and planetary centrifugal mixer. This hydrogel mixture slurry will shrink and compress the HAp powder during the sintering process. The porous bioceramics are expected to have good mechanical properties after sintering at 1200 degrees C. Reverse thermo-responsive hydrogels of poly[(N-isopropylacrylamide)-co-(methacrylic acid)] p(NiPAAm-MAA) were synthesized by free-radical cross-linking copolymerization, and their chemical properties were evaluated by nuclear magnetic resonance spectroscopy, infrared spectroscopy, and electrospray-ionization mass spectrometry. The lower critical solution temperature (LCST) of the hydrogel was determined using turbidity measurements. A thermogravimetric analysis was used to examine the thermal properties. The porous bioceramic properties were analyzed by X-ray diffraction, scanning electron microscopy, bulk density, compressive strength testing and cytotoxicity. The compressive strength and average porosity of the porous bioceramics were examined at approximately 6.8 MPa and 66% under 10 wt% p(NiPAAm-MAA)=99:1 condition. The ratio of HAp/beta-TCP can adjust two different compositional behaviors during the 1200 degrees C sintering process without resulting in cell toxicity. The (rhBMP-2)-HAp-PLGA carriers were fabricated as in our previous study of the double emulsion and drop-coating technique. Results of animal study included histological micrographs of the 1-mm defect in the femurs, with the rhBMP-2 carrier group, the bioceramic spacer group and the bioceramic spacer with rhBMP-2,carriers group showing better callus formation around the femur defect site than the control group. The optimal dual effects of the bone growth factors from osteoconductive bioceramics and osteoinductive rhBMP-2 carriers produced better bone formation. (C) 2013 The Authors. Published by Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据