期刊
JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY
卷 53, 期 2, 页码 119-126出版社
KOREAN SOC APPLIED BIOLOGICAL CHEMISTRY
DOI: 10.3839/jksabc.2010.021
关键词
Absidia corymbifera DY-9; chitin; chitin deacetylase; chitosan; enzymatic deacetylation
资金
- Korea Science and Engineering Foundation (KOSEF) through the National Research Lab
- Ministry of Science and Technology [M10300000322-06J0000-32210]
Recent attention has been paid to the screening of chitin deacetylase (CDA) from fungi, which may be applied into industrial production of chitosan and chitosan oligomers. We screened an extracellular CDA from a fungus that was further identified to be Absidia corymbifera DY-9. CDA from A. corymbifera DY-9 exhibited a higher enzyme activity than that of Mucor rouxii CDA. The highest yield of CDA was achieved in a liquid medium containing 2% powder chitin at pH 9.0, 28 degrees C and 150 rpm. Moreover, the biochemical properties of this enzyme were studied. It was found that the optimal pH and temperature were 6.5 and 55 degrees C. The enzyme activity could be enhanced in the presence of 1-10 mM Co2+, Ca2+ and Mg2+ while inhibited by acetate and EDTA. The enzyme was active on WSCT-50, glycol chitin, chitosan (DDA 71-88 %) and chitin oligosaccharides with a DP no less than 2.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据