Isolation of a Calcium-binding Peptide from Enzymatic Hydrolysates of Porcine Blood Plasma Protein

A calcium-binding peptide was purified from hydrolysates of porcine plasma protein. Porcine plasma protein was hydrolyzed using Flavourzyme, and the hydrolysates were filtered using YM-3 membrane. The membrane filtered solution was fractionated using Sephadex G-15, normal phase high-performance liquid, ion exchange chromatography, and fast protein liquid chromatography for a calcium-binding peptide. The sequence of the purified calcium-binding peptide was analyzed using liquid chromatography/electron spray ionization tandem mass spectrum and identified to be Val-Ser-Gly-Val-Glu-Asp-Val-Asn. The calcium- binding peptide can be used as a food ingredient or feed for calcium deficiency.