期刊
JOURNAL OF THE IRANIAN CHEMICAL SOCIETY
卷 7, 期 4, 页码 1004-1011出版社
SPRINGER
DOI: 10.1007/BF03246097
关键词
Hydrochlorothiazide; Molecularly imprinted polymer; Solid-phase extraction; Preconcentration
资金
- Research Affairs Division, Research Council and Center of Excellency in Sensor and Green Chemistry of Isfahan University of Technology (IUT)
A new polymeric sorbent prepared by utilizing molecular imprinting technology was used for the selective extraction of hydrochlorothiazide (HCT) from pharmaceutical and human serum sample. The molecularly imprinted polymer (MIP) was prepared using HCT as the template, methacrylic acid (MAA) as the functional monomer, ethylene glycol dimethacrylate (EDMA) as the cross-linker monomer, and dimethylformamide (DMF) as a solvent. The optimized conditions of MIPs as a selective sorbent for the preconcentration of the HCT were studied. The results showed that the drug could be quantitatively and selectively maintained in the column to be then eluted from the sorbent by using methanol-acetic acid mixture (9: 1). HCT could be determined spectrophotometrically at lambda(max) = 270 nm. This method made it possible to quantitize HCT in the range of 0.1-21.0 mu g ml(-1), by less than 0.55% of RSD%, with a detection limit (S/N = 3) of 0.073 ng ml(-1). The preconcentration factor of 1000, recoveries of up to 96% and retention capacity of 75.0 mg g(-1) were achieved using this technique.
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