期刊
JOURNAL OF THE INSTITUTE OF BREWING
卷 118, 期 2, 页码 243-248出版社
INST BREWING
DOI: 10.1002/jib.28
关键词
fungal community; Qingshuang-type Chinese rice wine; RISA; wheat Qu
资金
- Major State Basic Research Development Program of China (973 Program) [2012CB720802]
- National Natural Science Foundation of China [31171736]
- Program for New Century Excellent Talents in University [NCET-08-0790]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
A traditional culture-dependent method and ribosomal intergenic spacer analysis (RISA) were employed to investigate the fungal communities in two representative samples of wheat Qu (S1, S2). A total of seven species were isolated on media of potato dextrose agar, Czapek and wheat Qu extraction. Owing to the difference in the culture media, a high variability of predominant species was observed. RISA profiles of total DNA exhibited distinguishable bands corresponding to nine fungal species. Similar RISA profiles of two starter cultures were obtained. Sequencing of the internal transcribed spacer fragments from fungal isolates and RISA fingerprint profiles revealed the presence of nine species of fungi in wheat Qu S1: Lichtheimia ramosa, Absidia corymbifera, Aspergillus sydowii, Aspergillus oryzae, Rhizomucor pusillus, Eurotium amstelodami, Issatchenkia orientalis, Emericella nidulans and Cladosporium cladosporioides. In comparison with S1, S2 had a richer fungal diversity. The fungal species derived from S1 were all present in S2 coupled with another two species, namely, Aspergillus niger and Clavispora lusitaniae. Among all species isolated, Aspergillus sydowii was the dominant species in Qingshuang-type Chinese rice wine wheat Qu. Copyright (C) 2012 The Institute of Brewing & Distilling
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据