期刊
JOURNAL OF THE EUROPEAN OPTICAL SOCIETY-RAPID PUBLICATIONS
卷 8, 期 -, 页码 -出版社
EUROPEAN OPTICAL SOC
DOI: 10.2971/jeos.2013.13078
关键词
Confocal microscopy; hyperspectral imaging; spectroscopy; cell apoptosis; statistical methods
类别
资金
- IDEAS-FIRB [RBID08W2TR]
- AIRC
Among the optical techniques used for exploring the properties of cells and tissues, those based on hyperspectral label-free analysis are particularly interesting due to their non-invasive character and their ability to fast collect a huge number of information on the different sample constituents and their spatial distribution. Here we present results obtained with a novel hyperspectral reflectance confocal microscope of label-free discrimination of cells undergoing apoptosis. Our data, analyzed by means of a powerful statistical method, enable to obtain information on the biological status at a single cell level through the local measurement of reflectivity. Furthermore, an optical model of the local dielectric response gives an additional insight of the parameters linking the optical responsivity to the biological status.
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