期刊
JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY
卷 25, 期 3, 页码 317-327出版社
WILEY
DOI: 10.1111/j.1468-3083.2010.03792.x
关键词
hyaluronan; inter-alpha-inhibitor; keloid; scarring; TSG-6
类别
资金
- Arthritis Research UK [16539] Funding Source: Medline
- Biotechnology and Biological Sciences Research Council Funding Source: Medline
- Medical Research Council [76445] Funding Source: Medline
- Wellcome Trust Funding Source: Medline
Background Hyaluronan (HA) is a major component of the extracellular matrix (ECM) with increased synthesis during tissue repair. Tumour necrosis factor-stimulated gene-6 (TSG-6) is known to catalyze the covalent transfer of heavy chains (HC1 and HC2) from inter-alpha-inhibitor (I alpha I) onto HA, and resultant HC.HA complexes have been implicated in physiological and pathological processes related to remodelling and inflammation. Objective The aims of this study were to determine the expression of HA, TSG-6 and the IaI polypeptides in unscarred skin, normal scars and keloid scars. Methods Formalin-fixed paraffin-embedded sections of unscarred skin, normal scars and keloid scars were prepared from patient samples collected during scar revision surgery. Haematoxylin and eosin, as well as immunofluorescent staining for HA, TSG-6 and the three polypeptide chains of I alpha I (i.e. HC1, HC2 and bikunin) were performed. Results All skin types stained positive for TSG-6, HC1, HC2 and bikunin, associated with keratinocytes, fibroblasts and skin appendages all in close proximity to HA. Keloid lesions showed altered HA organization patterns compared with unscarred skin and normal scars. TSG-6 staining was significantly more intense in the epidermis compared with the dermis of all sample types. There was a significant reduction in TSG-6 levels within keloid lesions compared with the dermis of unscarred skin (P = 0.017). Conclusion TSG-6 is expressed in unscarred skin, where its close association with HA and I alpha I could give rise to TSG-6-mediated HC.HA formation within this tissue. A reduction in the beneficial effects of TSG-6, caused by diminished protein levels in keloid lesions, could contribute to this abnormal scarring process.
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