期刊
JOURNAL OF THE CHINESE MEDICAL ASSOCIATION
卷 77, 期 6, 页码 290-301出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1016/j.jcma.2014.01.007
关键词
acute liver damage; antioxidant activity; antityrosinase activity; D-galactosamine; phloretin; phloretin isonicotinyl hydrazone
资金
- National Natural Sciences Foundation of China [20962014]
- Natural Sciences Foundation of Jiangxi [2012ZBAB205010]
- Traditional Chinese Medicine Sciences Foundation of Jiangxi [2012A038]
- Sciences Foundation of Jiangxi University of Traditional Chinese Medicine [2012ZR017]
Background: Acute liver damage is primarily induced by one of several causes, among them viral exposure, alcohol consumption, and drug and immune system issues. Agents with the ability to inhibit tyrosinase and protect against DNA damage caused by reactive oxygen species (ROS) may be therapeutically useful for the prevention or treatment of ROS-related diseases. Methods: This investigation examined the hepatoprotective effects of phloretin and phloretin isonicotinyl hydrazone (PIH) on e-galactosamine (D-GalN)-induced acute liver damage in Kunming mice, as well as the possible mechanisms. The serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transferase (gamma-GT), alkaline phosphatase (ALP), and total bilirubin (TB) as well as the histopathological changes in mouse liver sections were determined The antioxidant effects of phloretin, quercetin, and PIH on lipid peroxidation in rat liver mitochondria in vitro, 1,1-dipheny1-2-picrylhydrazyl (DPPH) or 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) free radical scavenging activity in vitro, and supercoiled pBR322 plasmid DNA were confirmed. The experiment also examined the antityrosinase activity, inhibition type, and inhibition constant of phloretin and PIH. Results: Phloretin, quercetin, or PIH significantly prevented the increase in serum ALT, AST, gamma-GT, ALP, and TB in acute liver damage induced by D-GalN, and produced a marked reduction in the histopathological hepatic lesions. Phloretin, quercetin, or PIH also exhibited antioxidant effects on lipid peroxidation in rat liver mitochondria in vitro, DPPH or ABTS free radical scavenging activity in vitro, and supercoiled pBR322 plasmid DNA. Phloretin, quercetin, or PIH also exhibited good antityrosinase activity. Conclusion: To the best of our knowledge, this was the first study of the hepatoprotective effects of phloretin and PIH on D-GalN-induced acute liver damage in Kunming mice as well as the possible mechanisms. This was also the first study of the lipid peroxidation inhibition activity of phloretin and PIH in liver mitochondria induced by the Fe2+/vitamin C (Vc) system in vitro, the protective effects on supercoiled pBR322 plasmid DNA, and the antityrosinase activity of phloretin and PIH. Copyright (C) 2014 Elsevier Taiwan LLC and the Chinese Medical Association. All rights reserved.
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