Carbon Nanotubes Coated Fiber for Solid-phase Microextraction of Bovine Fibrinogen and Bovine Serum Albumin

Carbon nanotubes (CNTs) are a kind of novel and interesting carbon material which can be used for separation and purification. In this investigation, commercial solid-phase microextraction (SPME) fibers (PDMS) were coated with single-wall nanotubes (SWNTs) and multi-wall nanotubes (MWNTs) to study their adsorption and extraction ability of proteins, and bovine fibrinogen (BFg) and bovine serum albumin (BSA) were selected as the target proteins. While MWNTs adsorbed more BFg than SWNTs, SWNTs adsorbed more BSA than MWNTs. CNTs can selectively adsorb BFg in certain conditions. The fibers coated with CNTs had advantages over traditional SPME fibers in selectivity and sensitivity. It could be used to separate BFg in bovine blood plasma and also purify BFg from it. The results show that the selectivity, sensitivity and reproducibility of this method are good for real sample analysis.