期刊
JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY
卷 24, 期 12, 页码 1926-+出版社
SOC BRASILEIRA QUIMICA
DOI: 10.5935/0103-5053.20130240
关键词
cyclodipeptides; diketopiperazines; metagenomics; marine sponge
资金
- China Scholarship Council
- Mitsubishi Foundation
- Strategic Research Foundation Grant-aided Project for Private Universities
- Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan
- National Natural Science Foundation of China [11172337]
Culture-independent metagenomics is an attractive and promising approach to explore unique bioactive small molecules from marine sponges harboring uncultured symbiotic microbes. Therefore, we conducted functional screening of the metagenomic library constructed from the Japanese marine sponge Discodermia calyx. Bioassay-guided fractionation of plate culture extract of antibacterial clone pDC113 afforded eleven cyclodipeptides: Cyclo(L-Thr-L-Leu) (1), Cyclo(L-Val-D-Pro) (2), Cyclo(L-Ile-D-Pro) (3), Cyclo(L-Leu-L-Pro) (4), Cyclo(L-Val-L-Leu) (5), Cyclo(L-Leu-L-Ile) (6), Cyclo(L-Leu-L-Leu) (7), Cyclo(L-Phe-L-Tyr) (8), Cyclo(L-Trp-L-Pro) (9), Cyclo(L-Val-L-Trp) (10) and Cyclo(L-Ile-L-Trp) (11). To the best of our knowledge, these are first cyclodepeptides isolated from metagenomic library. Sequence analysis suggested that isolated cyclodipeptides were not synthesized by nonribosomal peptide synthetases and there was no significant indication of cyclodipeptide synthetases.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据