期刊
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
卷 24, 期 4, 页码 559-572出版社
AMER SOC NEPHROLOGY
DOI: 10.1681/ASN.2012080871
关键词
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资金
- National Institutes of Health [DK84077, DK87389, DK93493, DK94768, NCATS 1UH2 TR000504]
- American Heart Association [12040023]
- University of Washington Institute for Stem Cell and Regenerative Medicine
- Genzyme (Research in Progress Grant)
- NephCure Foundation
- Regulus Therapeutics
Microvascular disease, a characteristic of acute and chronic kidney diseases, leads to rarefaction of peritubular capillaries (PTCs), promoting secondary ischemic injury, which may be central to disease progression. Bidirectional signaling by EphB4 receptor and ephrinB2 ligand is critical for angiogenesis during murine development, suggesting that ephrinB2 reverse signaling may have a role in renal angiogenesis induced by injury or fibrosis. Here, we found that ephrinB2 reverse signaling is activated in the kidney only after injury. In mice lacking the PDZ intracellular signaling domain of ephrinB2 (ephrinB2 Delta V), angiogenesis was impaired and kidney injury led to increased PTC rarefaction and fibrosis. EphrinB2 Delta V primary kidney pericytes migrated more than wild-type pericytes and were less able to stabilize capillary tubes in three-dimensional culture and less able to stimulate synthesis of capillary basement membrane. EphrinB2 Delta V primary kidney microvascular endothelial cells migrated and proliferated less than wild-type microvascular endothelial cells in response to vascular endothelial growth factor A and showed less internalization and activation of vascular endothelial growth factor receptor-2. Taken together, these results suggest that PDZ domain-dependent ephrinB2 reverse signaling protects against PTC rarefaction by regulating angiogenesis and vascular stability during kidney injury. Furthermore, this signaling in kidney pericytes protects against pericyte-to-myofibroblast transition and myofibroblast activation, thereby limiting fibrogenesis. J Am Soc Nephrol 24: 559-572, 2013. doi: 10.1681/ASN.2012080871
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